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characteristic apoptotic morphological changes  (Beyotime)


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    Beyotime characteristic apoptotic morphological changes
    Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and <t>apoptotic</t> morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.
    Characteristic Apoptotic Morphological Changes, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 3202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/characteristic+apoptotic+morphological+changes/Hoechst+33258/pmc06151516-119-0-15
    Average 97 stars, based on 3202 article reviews
    characteristic apoptotic morphological changes - by Bioz Stars, 2026-10
    97/100 stars

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    1) Product Images from "Arenobufagin Induces Apoptotic Cell Death in Human Non-Small-Cell Lung Cancer Cells via the Noxa-Related Pathway"

    Article Title: Arenobufagin Induces Apoptotic Cell Death in Human Non-Small-Cell Lung Cancer Cells via the Noxa-Related Pathway

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules22091525

    Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and apoptotic morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.
    Figure Legend Snippet: Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and apoptotic morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.

    Techniques Used: Staining, Western Blot, Incubation, Trypan Blue Exclusion Assay

    Related Articles

    Staining:

    Article Title: Arenobufagin Induces Apoptotic Cell Death in Human Non-Small-Cell Lung Cancer Cells via the Noxa-Related Pathway
    Article Snippet: The optical density (OD) was measured at an absorbance wavelength of 490 nm, using a microplate reader (Thermo Fisher, Waltham, MA, USA). .. Characteristic apoptotic morphological changes were assessed by Hoechst 33258 staining, using the Hoechst staining kit (Beyotime, Haimen, China). ..



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    characteristic apoptotic morphological changes  (Beyotime)
    97
    Beyotime characteristic apoptotic morphological changes
    Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and <t>apoptotic</t> morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.
    Characteristic Apoptotic Morphological Changes, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/characteristic+apoptotic+morphological+changes/Hoechst+33258/pmc06151516-119-0-15
    Average 97 stars, based on 1 article reviews
    characteristic apoptotic morphological changes - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier

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    Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and apoptotic morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Arenobufagin Induces Apoptotic Cell Death in Human Non-Small-Cell Lung Cancer Cells via the Noxa-Related Pathway

    doi: 10.3390/molecules22091525

    Figure Lengend Snippet: Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and apoptotic morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.

    Article Snippet: Characteristic apoptotic morphological changes were assessed by Hoechst 33258 staining, using the Hoechst staining kit (Beyotime, Haimen, China).

    Techniques: Staining, Western Blot, Incubation, Trypan Blue Exclusion Assay